| Expression pattern: |
UP |
| Associated gene: |
RNA-splicing proteins, proteins involved in splicing, proteins involved in methylation, proteins involved in RNA transport, proteins involved in the cell cycle, proteins involved in cell junctions |
| Associated microRNA: |
hsa-miR-409-3p, hsa-miR-942-5p, hsa-miR-1276 |
| Biological function: |
Affects distant metastasis of colorectal cancer; potential diagnostic biomarker in CRC. |
| Molecular mechanism: |
Binds multiple RNA-binding proteins and binds miRNAs (hsa-miR-409-3p, hsa-miR-942-5p, hsa-miR-1276). |
| Biological pathway or process: |
migration (other); invasion (other); metastasis (promotes); cell cycle (other); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNase R Treatment; RT-qPCR; Sanger Sequencing; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis; Clinical Sample Validation; ROC Analysis |
| Clinical significance: |
circMSH3 is a potential biomarker for the diagnosis of CRC (AUC = 0.75; sensitivity 70.9%; specificity 66.4%) and its expression level is associated with distant metastasis (P = 0.043). |
| Description: |
circMSH3 (hsa_circ_0003761), derived from the MSH3 gene, is up-regulated in colorectal cancer tissues and CRC cell lines. Its expression is associated with distant metastasis and shows moderate diagnostic performance (AUC 0.75). Mechanistically, circMSH3 binds multiple RNA-binding proteins and directly binds hsa-miR-409-3p, hsa-miR-942-5p, and hsa-miR-1276. |
| Confidence score: |
0.7988 |