| Expression pattern: |
UP |
| Associated gene: |
FGB |
| Associated microRNA: |
miR-5580-5p |
| Biological function: |
promotes LUAD cell proliferation; promotes migration and invasion; promotes xenograft tumor growth; promotes my-CAF recruitment/formation in the TME |
| Molecular mechanism: |
ceRNA mechanism: circ_16601 sponges miR-5580-5p to stabilize/upregulate FGB mRNA, leading to Hippo/YAP1 pathway activation; exosomal circ_16601 promotes my-CAF formation |
| Biological pathway or process: |
Hippo/YAP (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (other); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Colony Formation Assay; Soft agar assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
high circ_16601 expression is positively associated with poor prognosis in LUAD patients |
| Description: |
circ_16601 is up-regulated in LUAD and promotes tumor proliferation, migration/invasion, and xenograft growth. It acts as a ceRNA by sponging miR-5580-5p to stabilize/upregulate FGB, which activates Hippo/YAP1 signaling; exosomal circ_16601 also promotes my-CAF recruitment/formation in the TME and is associated with poor prognosis. |
| Confidence score: |
0.8836 |