circRNA basic information
circBase ID: -
Name: hsa_circ_VAPA
Synonym: -
Host Gene: VAPA
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tumor mucosal tissues; paired normal mucosal tissues

Cell lines:

HCT116; LOVO; NCM460

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: CREB5
Associated microRNA: miR-125a
Biological function: circVAPA knockdown suppresses cell cycle progression, migration, invasion and glycolysis in colorectal cancer cells.
Molecular mechanism: Acts as a miR-125a sponge to positively regulate CREB5 expression.
Biological pathway or process:

cell cycle (inhibits); migration (inhibits); invasion (inhibits); glycolysis (inhibits); ceRNA regulation (other)

Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Cell Cycle Assay; Transwell Assay; Western Blot

Clinical significance:

-

Description:

circVAPA is up-regulated in colorectal cancer tissues and cell lines. Functionally, circVAPA promotes CRC cell cycle progression, migration/invasion and aerobic glycolysis. Mechanistically, circVAPA acts as a ceRNA by sponging miR-125a to increase CREB5 expression (circVAPA/miR-125a/CREB5 axis).

Confidence score:

0.7911

Other information
Title:

Circular RNA circVAPA knockdown suppresses colorectal cancer cell growth process by regulating miR-125a/CREB5 axis.

Journal: Cancer cell international
Published: 2020
PubMed ID: 32256212
Study type:

combined biological and clinical study

Data availability: The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -