| Expression pattern: |
UP |
| Associated gene: |
NEK4 |
| Associated microRNA: |
miR-485-3p |
| Biological function: |
circ-MEMO1 promotes LUAD cell viability, cell cycle progression, proliferation, migration and invasion under propofol treatment, and its overexpression attenuates propofol-induced anti-growth and anti-metastasis effects. |
| Molecular mechanism: |
circ-MEMO1 acts as a ceRNA that sponges miR-485-3p to regulate NEK4, thereby weakening propofol-induced tumor-suppressive effects in LUAD cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); drug resistance (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; EdU Staining; Cell Cycle Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-MEMO1 expression was associated with low 5-year overall survival and with tumor size, TNM stage, lymph node metastasis, and differentiation grade in LUAD patients. |
| Description: |
circ-MEMO1 is up-regulated in LUAD tissues and LUAD cell lines and high expression is linked to poorer overall survival. In vitro, circ-MEMO1 overexpression counteracts propofol-induced suppression of LUAD cell growth, migration and invasion by sponging miR-485-3p and regulating NEK4 within a ceRNA axis. |
| Confidence score: |
0.7126 |