| Expression pattern: |
UP |
| Associated gene: |
HDAC9, LRRC17 |
| Associated microRNA: |
let-7a-5p, miR-18a-3p, miR-126-5p, miR-146b-5p, miR-196b-5p |
| Biological function: |
promotes endothelial cell proliferation and survival; circBPTF silencing triggers cell cycle arrest and increases cell death |
| Molecular mechanism: |
ceRNA sponge mechanism: circBPTF binds multiple miRNAs (including miR-196b-5p) and indirectly regulates miRNA target mRNAs such as HDAC9 and LRRC17 |
| Biological pathway or process: |
cell cycle (inhibits); proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Transfection; Western Blot; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; Clinical Sample Validation |
| Clinical significance: |
circBPTF is upregulated in cardiac samples of end-stage IHF patients compared to healthy controls |
| Description: |
circBPTF is upregulated in ischemic heart failure LV tissue and induced by hypoxia in endothelial cells. It functions as a ceRNA hub binding multiple miRNAs (notably miR-196b-5p) to modulate target mRNAs such as HDAC9 and LRRC17. circBPTF inhibition suppresses endothelial proliferation and causes cell-cycle arrest with increased cell death. |
| Confidence score: |
0.7888 |