| Expression pattern: |
UP |
| Associated gene: |
WTAP, IGF2BP3, METTL3, METTL14 |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation, migration, invasion, and EMT in MNNG-induced malignant transformed gastric epithelial cells; promotes tumorigenesis in vivo. |
| Molecular mechanism: |
Activates TGFbeta/SMAD signaling; m6A modification mediated by WTAP; associated with increased IGF2BP3 level (IGF2BP3 predicted as binding protein). |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); m6A modification (other); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ0049271 is up-regulated in MNNG-induced malignant transformation of gastric epithelial cells and promotes proliferation, migration, invasion and EMT. It activates TGFbeta/SMAD signaling, and its m6A modification is proposed to be mediated by WTAP with an IGF2BP3-associated mechanism. |
| Confidence score: |
0.7006 |