circRNA basic information
circBase ID: hsa_circ_0005692
Name: hsa_circ_GLIS2
Synonym: circ_GLIS2 / circ_0005692
Host Gene: GLIS2
Genomic location(hg19): chr16:4382215-4383520:+
Genomic location(hg38): chr16:4332214-4333519:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

human HCC tissues; adjacent non-tumour liver tissues; lung tissues; tumours
Cell lines:

Huh-7; HepG2; HuH-6; BEL-100; BEL-7402; BEL-7405; HL-7702
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: GLIS2 mRNA, EIF4A3, DNMT1
Associated microRNA: -
Biological function: Suppresses HCC cell proliferation, migration, invasion, EMT, tumour growth and metastasis, and promotes cancer cell apoptosis.
Molecular mechanism: GLIS2 promoter hypermethylation reduces circ_GLIS2 expression in HCC; EIF4A3 directly binds GLIS2 mRNA transcript and promotes circ_GLIS2 and GLIS2 expression; circ_GLIS2 overexpression suppresses EMT-related marker changes.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); EMT (inhibits); metastasis (inhibits); mRNA stability (other); other pathway/process (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis; MSP (Methylation-Specific PCR)
Clinical significance:

Low circ_GLIS2 levels correlated with poor survival; circ_GLIS2 level discriminated advanced-stage from early-stage HCC patients and could serve as a diagnosis marker or therapeutic target for HCC.
Description:

circ_GLIS2 is a human GLIS2-derived circRNA that is down-regulated in HCC tissues and cell lines. Its overexpression suppresses HCC proliferation, migration, invasion, EMT, tumor growth and metastasis while promoting apoptosis, and its low expression is associated with poorer survival. Mechanistically, reduced circ_GLIS2 in HCC is linked to GLIS2 promoter hypermethylation, while EIF4A3 positively regulates circ_GLIS2/GLIS2 expression through binding the GLIS2 transcript.
Confidence score:

0.8961
Other information
Title:

Circular RNA circ_GLIS2 suppresses hepatocellular carcinoma growth and metastasis.

Journal: Liver international : official journal of the International Association for the Study of the Liver
Published: 2022
PubMed ID: 34743403
Study type:

combined biological and clinical study
Data availability: The datasets used or analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -