| Expression pattern: |
DN |
| Associated gene: |
RNF123, p27, pp27S10 |
| Associated microRNA: |
let-7i-5p, miR-29a-5p |
| Biological function: |
Promotes liver regeneration, hepatocyte proliferation, DNA replication, cell-cycle progression, bilirubin metabolism and resistance to surgery-related inflammation; circLRBA knockdown inhibits liver regeneration and hepatic cell growth. |
| Molecular mechanism: |
circLRBA acts as a scaffold that directly interacts with RNF123 and p27, reinforces RNF123-p27 binding, and facilitates RNF123-mediated ubiquitination-dependent degradation of p27/pp27S10, thereby restarting cell cycle progression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ubiquitination (promotes); inflammation (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; BrdU; Cell Cycle Assay; IHC (Immunohistochemistry); Western Blot; RNA-seq; In Vivo Animal Model; Cohort Study; Bioinformatics Analysis |
| Clinical significance: |
Baseline circLRBA levels in non-tumorous liver tissues are negatively associated with perioperative total bilirubin levels; circLRBA is down-regulated in cirrhotic liver tissues and may have therapeutic value for deficient cirrhotic liver regeneration. |
| Description: |
circLRBA is an LRBA-derived circRNA that is down-regulated in cirrhotic liver tissues but up-regulated during liver regeneration after partial hepatectomy. It promotes cirrhotic liver regeneration by acting as a scaffold for RNF123 and p27, facilitating ubiquitination-dependent p27 degradation and thereby enhancing cell-cycle progression, hepatocyte proliferation, liver metabolism and inflammatory resistance. Clinically, higher baseline circLRBA levels in non-tumorous liver tissue are associated with lower perioperative total bilirubin levels. |
| Confidence score: |
0.8972 |