| Expression pattern: |
DN |
| Associated gene: |
YTHDC1, TRAF6, IRAK1, Ago2 |
| Associated microRNA: |
miR-146a |
| Biological function: |
Promotes keratinocyte inflammatory response induced by Cutibacterium acnes biofilm; increases IL-6, IL-8, and TNF-alpha expression; facilitates NF-kappaB-p65 nuclear translocation. |
| Molecular mechanism: |
YTHDC1 binds m6A-modified hsa_circ_0102678 and promotes its nuclear export to the cytoplasm; cytoplasmic hsa_circ_0102678 functions as a ceRNA sponging miR-146a to upregulate IRAK1 and TRAF6. |
| Biological pathway or process: |
NF-kappaB (promotes); MAPK (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Back-Splice Junction PCR / divergent primers PCR; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Low expression of hsa_circ_0102678 in acne tissues may serve as a biomarker for auxiliary diagnosis of acne and a potential drug target. |
| Description: |
In acne, hsa_circ_0102678 is down-regulated in lesional tissues but promotes C. acnes biofilm-induced inflammatory responses in human primary keratinocytes. Mechanistically, YTHDC1 mediates m6A-dependent nuclear export of hsa_circ_0102678, and cytoplasmic hsa_circ_0102678 sponges miR-146a to upregulate IRAK1 and TRAF6, enhancing MAPK/NF-kappaB-associated inflammation. |
| Confidence score: |
0.7859 |