| Expression pattern: |
UP |
| Associated gene: |
STAT1 |
| Associated microRNA: |
miR-1296 |
| Biological function: |
promotes M1 macrophage polarization, increases the M1/M2 ratio, alleviates cisplatin resistance in gastric cancer cells, reduces GC cell viability under cisplatin treatment, and increases cisplatin-induced apoptosis |
| Molecular mechanism: |
circSOD2 acts as a microRNA sponge for miR-1296 and regulates STAT1 expression through the miR-1296/STAT1 axis, thereby promoting M1 macrophage polarization and enhancing cisplatin sensitivity |
| Biological pathway or process: |
ceRNA regulation (promotes); macrophage polarization (promotes); immune regulation (promotes); apoptosis (promotes); drug resistance (inhibits); chemoresistance (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; ELISA; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
CircSOD2 may serve as a potential target for GC chemotherapy and may be an applicable diagnostic or prognostic biomarker for immune therapy |
| Description: |
CircSOD2 is up-regulated in M1 macrophages and promotes M1 macrophage polarization in gastric cancer-related macrophage models. Mechanistically, cytoplasmic circSOD2 sponges miR-1296 to regulate STAT1, increasing the M1/M2 ratio and enhancing cisplatin-induced apoptosis while reducing cisplatin resistance in gastric cancer cells. |
| Confidence score: |
0.8278 |