| Expression pattern: |
DN |
| Associated gene: |
RPS3, SIRT1 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits hepatocyte inflammation and alleviates anti-tuberculosis drug-induced liver injury. |
| Molecular mechanism: |
hsa_circ_0093884 binds the RNA-binding protein RPS3 and activates/increases SIRT1 expression, thereby inhibiting inflammatory responses involving NLRP3, IL-1beta, and caspase-1. |
| Biological pathway or process: |
inflammation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; H&E Staining; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0093884 is decreased in serum samples of patients with ADLI and may be used to develop novel strategies for ADLI treatment. |
| Description: |
hsa_circ_0093884 is a SIRT1-derived human circRNA that is down-regulated in serum samples from ADLI patients and in an in vitro ADLI hepatocyte model. It binds the RNA-binding protein RPS3 to activate/increase SIRT1 expression, thereby suppressing NLRP3 inflammasome-related inflammatory responses and alleviating hepatocyte injury. |
| Confidence score: |
0.813 |