circRNA basic information
circBase ID: hsa_circ_0000081
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr1:64474976-64516388:+
Genomic location(hg38): chr1:64009304-64050716:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: Gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal tissues
Cell lines:

MKN-28 (MKN-74); MKN-45; AGS; MGC-803; GES-1; 293 T-cells
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: PDPK1, AGO2
Associated microRNA: miRNA-423-5p
Biological function: Promotes GC cell proliferation, migration, and invasion; associated with shorter survival time.
Molecular mechanism: Acts as a miRNA sponge for miR-423-5p to regulate PDPK1 expression (ceRNA-like regulation).
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; Bioinformatics Analysis; Survival Analysis; Clinical Sample Validation
Clinical significance:

Higher circRNA-0000081 expression is associated with shorter patient survival time.
Description:

hsa_circ_0000081 is up-regulated in gastric cancer tissues and cell lines and is associated with shorter patient survival. It promotes GC cell proliferation, migration, and invasion by acting as a cytoplasmic sponge for miR-423-5p, thereby increasing PDPK1 expression.
Confidence score:

0.8484
Other information
Title:

Hsa_circ_0000081 promotes the function of gastric cancer through sponging hsa-miR-423-5p to influence 3-phosphoinositide-dependent kinase 1 expression.

Journal: Bioengineered
Published: 2022
PubMed ID: 35302432
Study type:

combined biological and clinical study
Data availability: All data are included in the article; Supplemental data for this article can be accessed here
Code availability: -