| Expression pattern: |
UP |
| Associated gene: |
AZGP1, c-Met, G6PD, mTOR, SREBP1, YY1, HuR, AGO2, NOLC1 |
| Associated microRNA: |
miR-212-5p |
| Biological function: |
promotes colorectal cancer cell proliferation, migration, and liver metastasis; promotes oxPPP reprogramming and increases G6PD activity; supports NADPH production and reduces ROS |
| Molecular mechanism: |
Interacts with AZGP1 to activate mTOR/SREBP1 signaling; sponges miR-212-5p to upregulate c-Met, inducing G6PD and activating oxPPP; regulated by YY1; stabilizes host gene NOLC1 mRNA via HuR |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); oxidative phosphorylation (other); mitochondrial function (other); proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); circRIP; CLIP; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; EdU Staining; In Vivo Animal Model; H&E Staining; Western Blot; RNA-seq; Bioinformatics Analysis; ChIP / ChIP-seq; Oil Red O Staining |
| Clinical significance: |
circNOLC1 signal was increased in liver metastasis compared with corresponding primary CRC and was correlated with higher SREBP1, G6PD and c-Met and lower miR-212-5p in human specimens |
| Description: |
circNOLC1 is up-regulated in CRC, especially in liver metastasis, and promotes CRC cell proliferation, migration, and liver metastatic colonization. Mechanistically, circNOLC1 binds AZGP1 to activate mTOR/SREBP1 signaling and also sponges miR-212-5p to increase c-Met, converging on G6PD activation and oxidative PPP reprogramming with increased NADPH/ROS buffering. |
| Confidence score: |
0.8665 |