| Expression pattern: |
UP |
| Associated gene: |
IRAK1, AGO2 |
| Associated microRNA: |
miR-146a-5p |
| Biological function: |
promotes HNSCC cell proliferation, migration and invasion; promotes xenograft tumor growth; promotes EMT |
| Molecular mechanism: |
ceRNA/miRNA sponge: circCCT2 binds miR-146a-5p to upregulate IRAK1, promoting EMT and malignant progression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA-seq; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circCCT2 levels were associated with advanced T stage, N stage, clinical stage and poor prognosis. |
| Description: |
circCCT2 (hsa_circ_0000418) is up-regulated in HNSCC tissues and cell lines and mainly localizes to the cytoplasm. It promotes HNSCC proliferation, migration/invasion and xenograft growth by sponging miR-146a-5p to upregulate IRAK1 and drive EMT, and high circCCT2 expression is associated with advanced stage and poor overall survival. |
| Confidence score: |
0.8836 |