| Expression pattern: |
UP |
| Associated gene: |
METTL3, survivin, Ago2 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
promotes osteosarcoma cell proliferation, migration, invasion and tumor growth |
| Molecular mechanism: |
METTL3-mediated m6A modification enhances circRNF220; circRNF220 acts as a sponge for miR-330-5p to upregulate survivin |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
high expression of circRNF220 was associated with TNM stage and tumor size |
| Description: |
circRNF220 is up-regulated in osteosarcoma tissues and cell lines and promotes proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, METTL3-mediated m6A modification increases circRNF220, which sponges miR-330-5p to upregulate survivin. |
| Confidence score: |
0.8817 |