| Expression pattern: |
UP |
| Associated gene: |
CCND1, P53, P21 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
Promotes AVIC calcification, calcium nodule formation, osteogenic differentiation, ALP activity, and expression of osteogenic differentiation-related proteins and CCND1/P53/P21 pathway proteins. |
| Molecular mechanism: |
circ-CCND1 acts as a sponge for miR-138-5p to regulate CCND1 expression and activate the CCND1/P53/P21 signaling pathway. |
| Biological pathway or process: |
p53 signaling (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; Luciferase Reporter Assay; RNA Pull-Down; Western Blot |
| Clinical significance: |
circ-CCND1 can be used as a diagnostic marker for CAVD and may be a molecular target for the treatment of CAVD. |
| Description: |
circ-CCND1 is up-regulated in calcified aortic valve leaflets and promotes calcification and osteogenic differentiation of valve interstitial cells. Mechanistically, it sponges miR-138-5p to regulate CCND1 and activate the CCND1/P53/P21 pathway, suggesting potential diagnostic and therapeutic relevance in CAVD. |
| Confidence score: |
0.6675 |