| Expression pattern: |
UP |
| Associated gene: |
METTL14, AKT1S1, EIF4B, Ago2 |
| Associated microRNA: |
miR-30c-2-3p |
| Biological function: |
promotes proliferation and invasion (tumor-promoting effects) in gastric cancer cells |
| Molecular mechanism: |
METTL14-mediated m6A modification decreases circORC5 expression; circORC5 sponges miR-30c-2-3p to regulate AKT1S1 and EIF4B |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes); m6A modification (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; MeRIP / MeRIP-seq; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Colony Formation Assay; Transwell Assay; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circORC5 high expression indicated a poor survival in GC |
| Description: |
In gastric cancer, circORC5 is up-regulated and mainly localized in the cytoplasm. It promotes proliferation and invasion by sponging miR-30c-2-3p, thereby increasing downstream targets including AKT1S1 (and EIF4B); METTL14-mediated m6A modification reduces circORC5 expression and suppresses GC progression. |
| Confidence score: |
0.84 |