| Expression pattern: |
UP |
| Associated gene: |
AMOTL1, Ago2 |
| Associated microRNA: |
miR-485-5p |
| Biological function: |
promotes cervical cancer cell growth, proliferation and migration in vitro and promotes tumor growth in vivo |
| Molecular mechanism: |
acts as a ceRNA to upregulate AMOTL1 by sponging miR-485-5p (Ago2-dependent RISC association supported by RIP), forming the circAMOTL1/miR-485-5p/AMOTL1 axis |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
B
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Northern Blot; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Cohort Study; Clinical Sample Validation |
| Clinical significance: |
High circAMOTL1 expression is associated with poor prognosis and worse survival rates in cervical cancer patients; higher in metastatic tumors and higher clinical stage. |
| Description: |
circAMOTL1 (hsa_circ_0004214) is upregulated in cervical cancer and correlates with poorer overall survival. Functionally, it promotes proliferation and migration in cervical cancer cells and accelerates xenograft tumor growth. Mechanistically, circAMOTL1 acts as a ceRNA that sponges miR-485-5p to upregulate AMOTL1. |
| Confidence score: |
0.892 |