| Expression pattern: |
UP |
| Associated gene: |
BBC3, TEAD3, WNT9A, LLGL1, BMP8A |
| Associated microRNA: |
miR-503 |
| Biological function: |
Promotes cisplatin resistance (reduces cisplatin sensitivity) in ESCC cells. |
| Molecular mechanism: |
Predicted ceRNA mechanism: circ_0004365 may sponge miR-503 and participate in a circRNA-miR-mRNA network; Hippo signaling pathway genes were enriched by KEGG. |
| Biological pathway or process: |
chemoresistance (promotes); ceRNA regulation (promotes); Hippo/YAP (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; divergent primers PCR; RT-qPCR; Transfection; MTT |
| Clinical significance: |
Upregulation of circ_0004365 was associated with poor pathological response to chemotherapy and advanced pT stage after NAC, suggesting potential as a clinical predictor/biomarker of cisplatin sensitivity/resistance in ESCC. |
| Description: |
circ_0004365 (hsa_circ_0004365), derived from SEMA3C, is upregulated in cisplatin-resistant ESCC cell lines and in ESCC tumors after NAC. Its knockdown increases cisplatin sensitivity in vitro, and bioinformatic/RT-PCR evidence suggests it may act through a ceRNA network involving miR-503 and downstream targets/pathways (including Hippo pathway enrichment). |
| Confidence score: |
0.5495 |