| Expression pattern: |
UP |
| Associated gene: |
ASIC1, AGO2 |
| Associated microRNA: |
miR-185-3p, miR-939-5p |
| Biological function: |
promotes ROS-induced oxeiptosis and IVDD progression; increases susceptibility of NPCs to TBHP-induced cell death |
| Molecular mechanism: |
ceRNA (molecular sponge) of miR-185-3p and miR-939-5p to upregulate ASIC1, affecting ASIC1-mediated Ca2+ influx and activating the KEAP1-PGAM5-AIFM1 oxeiptosis-related pathway |
| Biological pathway or process: |
ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; TUNEL; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation |
| Clinical significance: |
targeted detection of circFOXO3 may help clinicians assess the progression status and treatment prognosis of IVDD |
| Description: |
CircFOXO3 is upregulated in degenerative nucleus pulposus tissue and in ROS-stimulated NPCs, and its knockdown suppresses oxeiptosis and alleviates IVDD in vivo. It acts as a ceRNA sponging miR-185-3p and miR-939-5p to increase ASIC1, enhancing ASIC1-mediated Ca2+ influx and activation of the KEAP1-PGAM5-AIFM1 oxeiptosis pathway. |
| Confidence score: |
0.8771 |