circRNA basic information
circBase ID: hsa_circ_0007376
Name: hsa_circ_MAP2K2
Synonym: circMAP2K2 / hsa_circRNA_102415
Host Gene: MAP2K2
Genomic location(hg19): chr19:4101016-4101278:-
Genomic location(hg38): chr19:4101018-4101280:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma; tumor tissue; normal mucosa tissues
Cell lines:

HGC-27; MKN-74; MKN-45; AGS; GSE-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PCBP1, EIF4A3, GPX1, PCBP2
Associated microRNA: -
Biological function: promotes proliferation, migration, invasion and metastasis of gastric cancer cells; induces EMT-like phenotype
Molecular mechanism: circMAP2K2 binds PCBP1 and promotes proteasome-mediated degradation of PCBP1, affecting GPX1 expression and activating AKT/GSK3beta signaling leading to EMT-like phenotype and enhanced malignancy; EIF4A3 promotes circMAP2K2 biogenesis/expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); PI3K/AKT (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

-
Description:

circMAP2K2 (hsa_circ_0007376) is up-regulated in gastric cancer cell lines and promotes proliferation and metastasis-related behaviors. Mechanistically, it binds PCBP1 in the cytoplasm and facilitates proteasome-mediated reduction of PCBP1, affecting GPX1 and activating AKT/GSK3beta signaling to drive an EMT-like phenotype; EIF4A3 contributes to its upregulation/biogenesis.
Confidence score:

0.8095
Other information
Title:

EGCG-LYS Fibrils-Mediated CircMAP2K2 Silencing Decreases the Proliferation and Metastasis Ability of Gastric Cancer Cells in Vitro and in Vivo.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Published: 2023
PubMed ID: 37752765
Study type:

combined biological and clinical study
Data availability: GSE93541; GSE83521
Code availability: -