| Expression pattern: |
DN |
| Associated gene: |
RSPO1 |
| Associated microRNA: |
miR-574-5p |
| Biological function: |
suppresses proliferation and inflammation and induces apoptosis of RA synovial fibroblasts (RASFs) |
| Molecular mechanism: |
acts as a miRNA sponge for miR-574-5p to up-regulate RSPO1 |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); inflammation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; Colony Formation Assay; EdU Staining; Western Blot; ELISA; Annexin V/PI Flow Cytometry; Bioinformatics Analysis |
| Clinical significance: |
circ_0000396 was reduced in serum of RA patients and suggested as having potential as a bio-marker |
| Description: |
circ_0000396 is down-regulated in serum from rheumatoid arthritis patients. In RASFs, circ_0000396 overexpression inhibits proliferation and inflammatory cytokine production and promotes apoptosis by sponging miR-574-5p to de-repress RSPO1. |
| Confidence score: |
0.7595 |