circRNA basic information
circBase ID: hsa_circ_0043256
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr17:35604934-35609962:-
Genomic location(hg38): chr17:37248010-37253036:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung adenocarcinoma tissues; para-carcinoma tissues; tumor tissues

Cell lines:

NCI-H460; A549

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DS
Associated gene: ITCH, Ago2, Wnt/beta-catenin pathway, Bcl-2, Bax, cleaved PARP, beta-catenin, c-Myc, CCND1
Associated microRNA: miR-1252
Biological function: hsa_circ_0043256 inhibits NSCLC cell proliferation, induces apoptosis, mediates cinnamaldehyde-induced apoptosis, and contributes to Wnt/beta-catenin pathway suppression.
Molecular mechanism: hsa_circ_0043256 acts as a miR-1252 sponge, relieves miR-1252-mediated repression of ITCH, upregulates ITCH, and thereby inhibits the Wnt/beta-catenin pathway in the anti-tumor response to cinnamaldehyde.
Biological pathway or process:

Wnt/beta-catenin (inhibits); apoptosis (promotes); proliferation (inhibits); ceRNA regulation (other); other pathway/process (other)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis

Clinical significance:

The hsa_circ_0043256/miR-1252/ITCH axis provides a novel insight into lung cancer therapy and implies the potential of cinnamaldehyde as a therapeutic agent against NSCLC.

Description:

This study identifies hsa_circ_0043256 as a cinnamaldehyde-responsive circular RNA in NSCLC models. hsa_circ_0043256 inhibits proliferation and promotes apoptosis by acting as a miR-1252 sponge, increasing ITCH expression and suppressing Wnt/beta-catenin signaling. Its knockdown weakens cinnamaldehyde-induced apoptosis and tumor-growth inhibition, supporting its role in cinnamaldehyde-mediated anti-tumor activity.

Confidence score:

0.7283

Other information
Title:

Cinnamaldehyde induces cell apoptosis mediated by a novel circular RNA hsa_circ_0043256 in non-small cell lung cancer.

Journal: Biochemical and biophysical research communications
Published: 2017
PubMed ID: 28958934
Study type:

biological research

Data availability: GSE101586; Supplemental table 1; Supplementary material
Code availability: -