| Expression pattern: |
UP |
| Associated gene: |
MDM2 |
| Associated microRNA: |
miR-1304-5p |
| Biological function: |
Promotes OS cell proliferation, migration and invasion, inhibits apoptosis, and contributes to OS cell development and progression in vitro. |
| Molecular mechanism: |
circ_0102049 acts as a ceRNA to sponge miR-1304-5p and upregulate MDM2 expression at the posttranscriptional level. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
High circ_0102049 expression was associated with larger tumor size, positive pulmonary metastasis, poor overall survival, and was an independent prognostic factor for OS patients after surgery. |
| Description: |
circ_0102049 is upregulated in osteosarcoma tissues and cell lines, and high expression is associated with larger tumor size, pulmonary metastasis, and poor overall survival. Functionally, circ_0102049 promotes OS cell proliferation, migration and invasion while suppressing apoptosis. Mechanistically, it acts as a cytoplasmic ceRNA that sponges miR-1304-5p to increase MDM2 expression. |
| Confidence score: |
0.7628 |