| Expression pattern: |
DN |
| Associated gene: |
BIRC5, SHCBP1, CCNA2, SKA3, GINS1 |
| Associated microRNA: |
hsa-miR-1183-5p, hsa-miR-1303-5p, hsa-miR-512-5p |
| Biological function: |
inhibits cell proliferation; inhibits cell-cycle progression |
| Molecular mechanism: |
Predicted as a ceRNA that binds miRNAs (hsa-miR-1183-5p, hsa-miR-1303-5p, hsa-miR-512-5p) to regulate downstream mRNAs in a circRNA-miRNA-mRNA network (bioinformatically constructed). |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Sanger Sequencing; Clinical Sample Validation; Transfection; EdU Staining; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0043256 may be a potential therapeutic target for lung cancer. |
| Description: |
hsa_circ_0043256 is significantly down-regulated in NSCLC tissues and cell lines. Overexpression inhibits NSCLC cell proliferation and cell-cycle progression in vitro, and a predicted ceRNA network was constructed linking hsa_circ_0043256 to miR-1183-5p/miR-1303-5p/miR-512-5p and downstream mRNAs (including hub genes such as BIRC5 and CCNA2). |
| Confidence score: |
0.6777 |