circRNA basic information
circBase ID: hsa_circ_0001681
Name: hsa_circ_RAPGEF5
Synonym: Circ-RAPGEF5 / CircRAPGEF5
Host Gene: RAPGEF5
Genomic location(hg19): chr7:22330793-22357656:-
Genomic location(hg38): chr7:22291174-22318037:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0003210
MONDO name: intrahepatic cholangiocarcinoma
Disease details: intrahepatic cholangiocarcinoma / ICC
Disease DO ID:
 4928
Disease MeSH ID:
 -
Disease NCIt ID:
 C35417
Disease ICD11 ID:
 1253728223; 387909164
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ICC tumor tissues; adjacent normal tissues
Cell lines:

RBE; CCLP1; 9810; HUCCT1; HIBEC
In vivo animal model:

patient-derived xenograft; cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SAE1, AKT
Associated microRNA: miR-3185
Biological function: Promotes ICC tumor proliferation and metastasis, and inhibits apoptosis.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-3185 to stabilize SAE1 mRNA, thereby promoting SUMOylation including AKT SUMOylation.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); metastasis (promotes); apoptosis (inhibits); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

circRNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; Co-IP; Western Blot; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High Circ-RAPGEF5 expression was significantly correlated with a poor prognosis in patients with ICC.
Description:

Circ-RAPGEF5 (hsa_circ_0001681), derived from RAPGEF5, is up-regulated in intrahepatic cholangiocarcinoma and promotes proliferation, migration/invasion, and metastasis while inhibiting apoptosis. Mechanistically, it sponges miR-3185 to stabilize SAE1, enhancing global SUMOylation and AKT SUMOylation. High Circ-RAPGEF5 expression is associated with poorer patient survival.
Confidence score:

0.8929
Other information
Title:

Circ-RAPGEF5 promotes intrahepatic cholangiocarcinoma progression by stabilizing SAE1 to facilitate SUMOylation.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2023
PubMed ID: 37705041
Study type:

combined biological and clinical study
Data availability: PRJCA018197
Code availability: -