circRNA basic information
circBase ID: -
Name: hsa_circ_Cul2
Synonym: circ-10720 / Cul2 circular RNA
Host Gene: Cul2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumor tissues; liver tumor tissues; fresh surgical HCC tumor tissues
Cell lines:

PLC-PRF-5; SMMC-7721; HEK-293T
In vivo animal model:

patient-derived xenograft; genetically engineered animal model; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: Twist1, Vimentin, Cul2 promoter
Associated microRNA: miR-1246, miR-127-5p, miR-331-5p, miR-1200, miR-888, miR-587, miR-578, miR-656, miR-890, miR-490-5p, miR-1238, miR-548g, miR-513a-3p, miR-521
Biological function: Promotes EMT, tumor growth, proliferation, migration, invasion, metastasis and HCC malignancy; increases Vimentin expression and decreases E-cadherin expression; knockdown counteracts Twist1-induced tumor-promoting effects.
Molecular mechanism: Twist1 activates Cul2 transcription and promotes circ-10720 formation; circ-10720 functions as a miRNA sponge to adsorb Vimentin-targeting miRNAs, thereby increasing Vimentin expression and promoting EMT.
Biological pathway or process:

EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); VEGF/VEGFR (promotes); TGF-beta/SMAD (promotes); ceRNA regulation (promotes); apoptosis (other)
Detected method:
Q
H
Validation methods:

RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; IF (Immunofluorescence); Western Blot; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); ELISA; Survival Analysis; Cohort Study; Bioinformatics Analysis
Clinical significance:

High circ-10720 expression is associated with metastasis, advanced clinical stage, higher pathological grade, AFP positivity, poor prognosis, and may serve as a potential HCC biomarker.
Description:

circ-10720 is a Cul2-derived circular RNA up-regulated in aggressive HCC and positively associated with Twist1, Vimentin, metastasis, advanced stage, and poor survival. Twist1 promotes circ-10720 formation, and circ-10720 acts as a miRNA sponge for Vimentin-targeting miRNAs, thereby increasing Vimentin expression, promoting EMT, proliferation, migration, invasion, tumor growth and metastasis. Its high expression may serve as a prognostic biomarker and therapeutic target in HCC.
Confidence score:

0.807
Other information
Title:

Twist1 Regulates Vimentin through Cul2 Circular RNA to Promote EMT in Hepatocellular Carcinoma.

Journal: Cancer research
Published: 2018
PubMed ID: 29844124
Study type:

combined biological and clinical study
Data availability: TCGA database; Supplementary Table S1; Supplementary Table S2; Supplementary Table S3; Supplementary Table S4; Supplementary Table S5
Code availability: -