| Expression pattern: |
UP |
| Associated gene: |
ADAM10, Ago2 |
| Associated microRNA: |
miR-499a-3p |
| Biological function: |
attenuates ox-LDL-induced endothelial cell apoptosis and promotes migration; supports cell viability |
| Molecular mechanism: |
ceRNA sponge mechanism: circ_0006867 sponges miR-499a-3p to regulate ADAM10 expression, affecting apoptosis and migration under ox-LDL-induced injury |
| Biological pathway or process: |
apoptosis (inhibits); migration (promotes); proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Wound Healing Assay; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In ox-LDL-treated HUVECs (an in vitro atherosclerosis injury model), circ_0006867 is up-regulated and mainly localized in the cytoplasm. It sponges miR-499a-3p to modulate ADAM10 expression, thereby reducing apoptosis and supporting endothelial cell migration/viability under ox-LDL-induced injury. |
| Confidence score: |
0.7626 |