| Expression pattern: |
UP |
| Associated gene: |
STIM1, AGO2, CaMKII, ERK1/2, FAK |
| Associated microRNA: |
miR-185-5p |
| Biological function: |
Promotes Ca2+ influx-mediated epithelial-to-mesenchymal transition of renal tubular epithelial cells and contributes to renal interstitial fibrosis; knockdown attenuates TGF-beta1-induced EMT-associated protein expression. |
| Molecular mechanism: |
CircRNA_0017076 acts as a ceRNA sponge for miR-185-5p, relieving repression of STIM1 and activating Ca2+/CaMKII/ERK/FAK signaling to promote EMT. |
| Biological pathway or process: |
ceRNA regulation (promotes); EMT (promotes); fibrosis (promotes); ERK (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Identified as a biomarker and potential therapeutic target for RIF disease in humans. |
| Description: |
hsa_circ_0017076/CircRNA_0017076 is up-regulated in human RIF kidney biopsies and TGF-beta1-treated HK-2 cells. It functions as a cytoplasmic miR-185-5p sponge to increase STIM1 expression, enhance Ca2+ influx, activate CaMKII/ERK/FAK signaling, and promote EMT and renal fibrosis, suggesting potential biomarker and therapeutic relevance. |
| Confidence score: |
0.8414 |