| Expression pattern: |
UP |
| Associated gene: |
YAP1, AMPK |
| Associated microRNA: |
miR-186-5p |
| Biological function: |
Silencing circZNF609 restrained PC cell colony formation, viability, migration and invasion, and enhanced apoptosis; therefore circZNF609 appears to promote growth, migration and invasion and inhibit apoptosis in prostate cancer cells. |
| Molecular mechanism: |
Silencing circZNF609 up-regulates miR-186-5p, and miR-186-5p inhibitor reverses the effects of circZNF609 silencing on cell growth, apoptosis, migration and invasion; circZNF609 also affects YAP1 and AMPK signaling pathways through miR-186-5p. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); Hippo/YAP (promotes); AMPK (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot |
| Clinical significance: |
circZNF609 was highly expressed in PC tissues and the authors suggested it may provide a new idea for clinical treatment of PC. |
| Description: |
circZNF609 is up-regulated in prostate cancer tissues. In PC3 and LNCaP cells, circZNF609 silencing inhibits proliferation/colony formation, migration and invasion while inducing apoptosis. Mechanistically, circZNF609 negatively regulates miR-186-5p and affects YAP1 and AMPK signaling pathways. |
| Confidence score: |
0.5434 |