| Expression pattern: |
UP |
| Associated gene: |
METTL3, SPTBN1, NLRP3 inflammasome |
| Associated microRNA: |
- |
| Biological function: |
Promotes colonic inflammation and Crohn's colitis by enhancing NLRP3 inflammasome-mediated pyroptosis and impairing/suppressing autophagy in colonic epithelial cells. |
| Molecular mechanism: |
METTL3-mediated m6A modification increases circPRKAR1B; circPRKAR1B binds the RBP SPTBN1, which impairs autophagy and promotes NLRP3 inflammasome-mediated pyroptosis. |
| Biological pathway or process: |
autophagy (inhibits); pyroptosis (promotes); inflammation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; MeRIP / MeRIP-seq; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Western Blot; IF (Immunofluorescence); ELISA; TUNEL; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circPRKAR1B expression is elevated in CD colon tissues and is closely correlated with CD-associated inflammatory indices (CRP, CDAI, SES-CD and faecal calprotectin). |
| Description: |
circPRKAR1B (hsa_circ_0008039) is upregulated in Crohn’s disease/colitis colon tissues, particularly in epithelial layers. METTL3-mediated m6A modification promotes its elevated expression, and circPRKAR1B binds SPTBN1 to inhibit autophagy and enhance NLRP3 inflammasome-mediated pyroptosis, aggravating intestinal inflammation. |
| Confidence score: |
0.8064 |