| Expression pattern: |
UP |
| Associated gene: |
hnRNPA1, AGO2, LRP5 |
| Associated microRNA: |
- |
| Biological function: |
promotes prostate cancer cell proliferation, migration, invasion, and tumor growth/metastasis |
| Molecular mechanism: |
IRES-dependent rolling circle translation of circSPIRE1 produces rtSPIRE1; translation is regulated by hnRNPA1 (SDMA-mediated) binding to IRES; functional output involves stabilization of LRP5 via inhibiting ubiquitination and activating PI3K/AKT/mTOR signaling |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
High circSPIRE1 expression correlates with higher Gleason scores, advanced stage/metastasis, and poorer prognosis |
| Description: |
circSPIRE1 is up-regulated in prostate cancer and promotes proliferation, migration and invasion. It is translated via an IRES-dependent rolling circle mechanism regulated by hnRNPA1 SDMA modification to generate rtSPIRE1, which stabilizes LRP5 by inhibiting ubiquitination and thereby activates PI3K/AKT/mTOR signaling and EMT. |
| Confidence score: |
0.8817 |