| Expression pattern: |
UP |
| Associated gene: |
PGF, YTHDF1, METTL3 |
| Associated microRNA: |
miR-342-5p |
| Biological function: |
promotes colorectal cancer cell migration and invasion and promotes metastasis |
| Molecular mechanism: |
m6A modification enhances circALG1 binding to miR-342-5p and promotes ceRNA function to upregulate PGF; YTHDF1-mediated m6A recognition contributes by enhancing circALG1 stability |
| Biological pathway or process: |
metastasis (promotes); migration (promotes); invasion (promotes); EMT (promotes); MAPK (promotes); ERK (promotes); ceRNA regulation (promotes); m6A modification (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Western Blot; RNA-seq; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; ROC Analysis |
| Clinical significance: |
circALG1 expression distinguished CRC tumours vs adjacent tissues and CRC peripheral blood vs healthy individuals (ROC analysis); high circALG1 expression associated with metastasis and higher CA19-9; proposed prognostic marker/therapeutic target |
| Description: |
circALG1 is up-regulated in colorectal cancer tissues and peripheral blood and promotes CRC metastasis by enhancing migration and invasion. Mechanistically, m6A-modified circALG1 (recognized by YTHDF1) sponges miR-342-5p to upregulate PGF, which activates ERK/MAPK and EMT-associated changes. |
| Confidence score: |
0.8861 |