| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
CircVPS35L is a stable peripheral blood-derived diagnostic biomarker for NSCLC; its low expression is correlated with tumor size, histology type, and TNM stage, and it can distinguish NSCLC from healthy controls and benign lung disease. |
| Molecular mechanism: |
circVPS35L is generated by backsplicing of VPS35L exons 23-26 and has a stable closed-loop circular RNA structure resistant to RNase R and with a longer half-life after actinomycin D treatment. |
| Biological pathway or process: |
not specified |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circVPS35L expression is associated with tumor size, histology type, and TNM stage; blood-derived circVPS35L has diagnostic value for NSCLC and can distinguish NSCLC from benign lung disease, with better diagnostic performance than CYFR21-1, NSE, CEA, or their combined panel. |
| Description: |
This study identifies hsa_circ_0003645/circVPS35L as a down-regulated circRNA in NSCLC tissues, cell lines, and peripheral blood. circVPS35L is a stable circular RNA derived from VPS35L exons 23-26 and shows diagnostic utility for distinguishing NSCLC from healthy controls and benign lung disease, outperforming conventional tumor markers in ROC analyses. |
| Confidence score: |
0.6841 |