circRNA basic information
circBase ID: hsa_circ_0050900
Name: hsa_circ_ACTN4
Synonym: circRNA actinin alpha 4 (ACTN4)
Host Gene: ACTN4
Genomic location(hg19): chr19:39191239-39200116:+
Genomic location(hg38): chr19:38700599-38709476:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0003210
MONDO name: intrahepatic cholangiocarcinoma
Disease details: intrahepatic cholangiocarcinoma / ICC
Disease DO ID:
 4928
Disease MeSH ID:
 -
Disease NCIt ID:
 C35417
Disease ICD11 ID:
 1253728223; 387909164
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HuCCT-1; QBC-939; HCCC-9810; RBE; 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: SLC3A2, AGO2
Associated microRNA: hsa-miR-605-3p
Biological function: Promotes proliferation and migration and inhibits ferroptosis in ICC cells.
Molecular mechanism: Acts as a ceRNA to sponge hsa-miR-605-3p, thereby regulating SLC3A2 and ferroptosis-related phenotypes.
Biological pathway or process:

ferroptosis (inhibits); proliferation (promotes); migration (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; Transwell Assay; Western Blot; Flow Cytometry（Non-apoptosis/cycle）
Clinical significance:

-
Description:

In ICC cell lines, hsa_circ_0050900 is upregulated and mainly localized in the cytoplasm. It sponges hsa-miR-605-3p to upregulate SLC3A2, thereby suppressing ferroptosis and promoting proliferation and migration.
Confidence score:

0.7551
Other information
Title:

Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2.

Journal: Oncology letters
Published: 2024
PubMed ID: 38028176
Study type:

biological research
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -