circRNA basic information
circBase ID: hsa_circ_0002690
Name: hsa_circ_LIMK1
Synonym: circLIMK1-005 / circLIMK1
Host Gene: LIMK1
Genomic location(hg19): chr7:73510951-73511519:+
Genomic location(hg38): chr7:74096621-74097189:+
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
3910
Disease MeSH ID:
C538231
Disease NCIt ID:
C3512
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

LUAD tissues; adjacent normal tissues

Cell lines:

A549; H1299; PC9; HCC827; bes-2b

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: RPA1, Cyclin D1, CDK4, phospho-RB, phospho-AKT
Associated microRNA: -
Biological function: promotes LUAD cell proliferation and metastasis; promotes tumor growth in vivo
Molecular mechanism: binds RPA1 protein and increases RPA1 protein level (post-transcriptional), elevating Cyclin D1 and CDK4 proteins to activate CDK4 signaling
Biological pathway or process:

proliferation (promotes); metastasis (promotes); cell cycle (promotes); PI3K/AKT (promotes)

Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; RNA-seq; Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis

Clinical significance:

elevated circLIMK1-005 expression is associated with poor prognosis in LUAD patients

Description:

circLIMK1-005 is up-regulated in LUAD tissues/cells, mainly localized in the nucleus, and its high expression associates with poor patient survival. It promotes LUAD proliferation, cell-cycle progression, migration/invasion, and tumor growth by binding RPA1, increasing RPA1 protein level, and activating Cyclin D1/CDK4 signaling (with AKT phosphorylation as a downstream readout).

Confidence score:

0.8766

Other information
Title:

Circular RNA circLIMK1-005 promotes the progression of lung adenocarcinoma by interacting with RPA1 protein to activate CDK4 signaling.

Journal: Cell death discovery
Published: 2025
PubMed ID: 40593477
Study type:

combined biological and clinical study

Data availability: The online version contains supplementary material available at 10.1038/s41420-025-02565-y.
Code availability: -