| Expression pattern: |
UP |
| Associated gene: |
PAK2 / AGO2 |
| Associated microRNA: |
hsa-miR-140-3p |
| Biological function: |
promotes proliferation, migration, invasion, EMT and angiogenesis; supports tumorigenesis and metastasis; exosomal circ_0001789 mediates malignant phenotype in recipient cells |
| Molecular mechanism: |
circ_0001789 acts as a miRNA sponge for miR-140-3p, thereby upregulating PAK2; associated with AGO2-containing RISC; exosomal transfer increases circ_0001789 in recipient cells |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); angiogenesis (promotes); ceRNA regulation (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Upregulation of circ_0001789 is associated with metastasis, advanced TNM stage, and poor prognosis; exosomal circ_0001789 shows diagnostic value (AUC > 0.8). |
| Description: |
circ_0001789 is up-regulated in gastric cancer tissues, cells, and serum exosomes and correlates with metastasis and poor overall survival. It promotes malignant phenotypes (proliferation, migration/invasion, EMT, angiogenesis, tumorigenesis/metastasis) largely by sponging miR-140-3p to upregulate PAK2. |
| Confidence score: |
0.8823 |