| Expression pattern: |
UP |
| Associated gene: |
RPLP0, AGO2, PI3K/Akt pathway |
| Associated microRNA: |
miR-582-3p |
| Biological function: |
circATP9A promotes NSCLC cell proliferation, invasion, migration, EMT and tumor growth, and suppresses apoptosis. |
| Molecular mechanism: |
circATP9A acts as a competitive endogenous RNA sponge for miR-582-3p, upregulates RPLP0 expression, and activates the PI3K/Akt signaling pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Northern Blot; FISH / smFISH; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circATP9A overexpression is associated with lymph node metastasis and TNM staging in NSCLC patients and may be an ideal diagnostic biomarker for NSCLC. |
| Description: |
circATP9A (hsa_circ_0008253) is up-regulated in NSCLC tissues and cell lines. It functions as an oncogenic circRNA by sponging miR-582-3p, increasing RPLP0 expression, activating PI3K/Akt signaling, and thereby promoting proliferation, invasion, migration, EMT and xenograft tumor growth while inhibiting apoptosis. Its high expression is associated with lymph node metastasis and TNM staging, suggesting potential clinical biomarker value. |
| Confidence score: |
0.8512 |