| Expression pattern: |
UP |
| Associated gene: |
OXSR1 |
| Associated microRNA: |
miR-339-5p |
| Biological function: |
CircNRIP1 promotes LPS-induced HK2 cell apoptosis and inflammation; circNRIP1 knockdown alleviates LPS-induced HK2 cell injury by improving cell viability and reducing apoptosis and inflammatory cytokine production. |
| Molecular mechanism: |
CircNRIP1 acts through a ceRNA-like circNRIP1/miR-339-5p/OXSR1 pathway, binding miR-339-5p and positively regulating OXSR1 expression. |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ELISA |
| Clinical significance: |
CircNRIP1 was upregulated in blood samples from septic AKI patients and may be a therapeutic target for AKI incurred by sepsis. |
| Description: |
CircNRIP1 is up-regulated in blood from septic AKI patients and in LPS-induced HK2 cells. The study shows that circNRIP1 knockdown protects HK2 cells from LPS-induced injury by reducing apoptosis and inflammatory cytokine production, mechanistically through the miR-339-5p/OXSR1 axis. |
| Confidence score: |
0.7968 |