| Expression pattern: |
UP |
| Associated gene: |
PDL1, programmed cell death receptor ligand 1 |
| Associated microRNA: |
miR-485-3p |
| Biological function: |
Promotes colorectal cancer cell growth, proliferation, migration, invasion, immune escape and tumor growth, while inhibiting apoptosis. |
| Molecular mechanism: |
circ-KRT6C functions as an miR-485-3p sponge to upregulate PDL1, thereby promoting malignant progression and immune evasion of colorectal cancer cells. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); immune regulation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; ELISA; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ-KRT6C level was associated with overall survival time of patients with colorectal cancer; high circ-KRT6C expression was associated with shorter survival, and circ-KRT6C was proposed as a new diagnosis target. |
| Description: |
circ-KRT6C is an up-regulated oncogenic circRNA in colorectal cancer tissues and cells, and high expression is associated with poorer overall survival. Mechanistically, circ-KRT6C sponges miR-485-3p to increase PDL1 expression, promoting proliferation, migration, invasion, immune evasion and xenograft tumor growth while suppressing apoptosis. |
| Confidence score: |
0.786 |