| Expression pattern: |
UP |
| Associated gene: |
CCL16, Ago2 |
| Associated microRNA: |
miR-141-3p |
| Biological function: |
circ_0044411 promotes LPS-induced MRC-5 cell injury, inflammatory response and apoptosis, and inhibits cell viability and proliferation; circ_0044411 knockdown protects MRC-5 cells from LPS-induced cell damage. |
| Molecular mechanism: |
circ_0044411 acts as a ceRNA sponge for miR-141-3p and positively regulates CCL16 expression, thereby regulating LPS-induced inflammatory injury. |
| Biological pathway or process: |
ceRNA regulation (promotes); inflammation (promotes); apoptosis (promotes); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot |
| Clinical significance: |
circ_0044411 was increased in serum of infantile pneumonia patients and might be a potential therapeutic target for IP. |
| Description: |
This study found that circ_0044411 is up-regulated in serum from infantile pneumonia patients and in LPS-induced MRC-5 cells. Functionally, circ_0044411 knockdown protects against LPS-induced cell injury by promoting viability and proliferation while inhibiting apoptosis and inflammatory cytokine responses. Mechanistically, circ_0044411 sponges miR-141-3p to positively regulate CCL16 expression, suggesting a circ_0044411/miR-141-3p/CCL16 ceRNA axis and potential therapeutic relevance in infantile pneumonia. |
| Confidence score: |
0.8275 |