| Expression pattern: |
UP |
| Associated gene: |
EGFR, Ago2 |
| Associated microRNA: |
hsa-miR-3184-5p |
| Biological function: |
promotes proliferation, migration, invasion, metastasis and chemoresistance; inhibits apoptosis; promotes cell cycle progression |
| Molecular mechanism: |
circEMB acts as a miR-3184-5p sponge in an Ago2-containing complex, thereby upregulating EGFR and influencing Notch signaling and MTX resistance |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes); Notch (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circEMB expression is associated with metastasis, advanced clinical stage, worse survival, and has ROC performance for distinguishing metastasis (AUC 0.703) |
| Description: |
circEMB is up-regulated in osteosarcoma tissues/cells and promotes proliferation, invasion/migration, metastasis, and MTX resistance. It mainly functions as a cytoplasmic ceRNA by sponging miR-3184-5p in an Ago2-associated complex, thereby increasing EGFR expression and impacting Notch signaling. High circEMB expression correlates with metastasis and poor prognosis. |
| Confidence score: |
0.8942 |