circRNA basic information
circBase ID: hsa_circ_0065173
Name: hsa_circ_SETD2
Synonym: circSETD2
Host Gene: SETD2
Genomic location(hg19): chr3:47155365-47168153:-
Genomic location(hg38): chr3:47113875-47126663:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissue samples; adjacent normal tissues
Cell lines:

MCF7; MDA-231; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: HuR, YAP1, CTGF, myc, SOX2, Slug
Associated microRNA: -
Biological function: Suppresses breast cancer cell proliferation, migration, invasion and EMT; inhibits tumor growth in vivo.
Molecular mechanism: circSETD2 competitively binds HuR, reduces HuR-YAP1 mRNA interaction and YAP1 mRNA stability, decreasing YAP1 and downstream targets (CTGF, myc, SOX2, Slug).
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); mRNA stability (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Sanger Sequencing; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

-
Description:

circSETD2 is down-regulated in breast cancer tissues/cells and acts as a tumor suppressor by inhibiting proliferation, migration, invasion and EMT. It binds the RBP HuR and competitively disrupts HuR-mediated stabilization of YAP1 mRNA, reducing YAP1 and downstream targets (e.g., CTGF, myc, SOX2, Slug), thereby suppressing breast cancer progression.
Confidence score:

0.8095
Other information
Title:

CircSETD2 inhibits YAP1 by interaction with HuR during breast cancer progression.

Journal: Cancer biology & therapy
Published: 2023
PubMed ID: 37606201
Study type:

combined biological and clinical study
Data availability: GSE101123; TCGA (https://portal.gdc.cancer.gov); https://doi.org/10.1080/15384047.2023.2246205
Code availability: -