circRNA basic information
circBase ID: hsa_circ_0001715
Name: hsa_circ_LIMK1
Synonym: circ_0001715
Host Gene: LIMK1
Genomic location(hg19): chr7:73521339-73523366:+
Genomic location(hg38): chr7:74107009-74109036:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paired normal tissues; xenograft tumor tissues
Cell lines:

BEAS-2B; A549; H1299; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CANT1
Associated microRNA: miR-1322
Biological function: promoted proliferation, migration, sphere formation and tube formation, and inhibited apoptosis in LUAD cells; promoted tumor growth in vivo
Molecular mechanism: ceRNA mechanism: circ_0001715 sponges miR-1322 to upregulate CANT1
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); angiogenesis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Colony Formation Assay; EdU Staining; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; Tube Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation
Clinical significance:

High circ_0001715 expression was associated with TNM stage and lymph node metastasis of LUAD patients; proposed as an effective biomarker
Description:

circ_0001715 is up-regulated in LUAD tissues and cell lines and promotes malignant phenotypes (proliferation, migration, sphere formation, and angiogenesis-related tube formation) while inhibiting apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-1322 to increase CANT1, and its knockdown suppresses xenograft tumor growth.
Confidence score:

0.8006
Other information
Title:

Circ_0001715 accelerated lung adenocarcinoma process by the miR-1322/CANT1 axis.

Journal: Diagnostic pathology
Published: 2023
PubMed ID: 37553672
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1186/s13000-023-01348-2. / The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -