| Expression pattern: |
UP |
| Associated gene: |
PAQR4, NF-kappaB |
| Associated microRNA: |
miR-647, miR-326 |
| Biological function: |
Promotes prostate cancer cell proliferation, migration, and tumor growth (in vitro and in vivo); promotes angiogenesis in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-647 to upregulate PAQR4 and activate PI3K/Akt signaling; NF-kappaB binds the NOLC1 promoter to upregulate NOLC1 and circNOLC1. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); angiogenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; RNA-seq; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
Potential biomarker and therapeutic target for PCa. |
| Description: |
circNOLC1 is upregulated in prostate cancer and functions as an oncogenic circRNA. It sponges miR-647 to increase PAQR4 expression and thereby promotes PI3K/AKT pathway activation, enhancing proliferation, migration, tumor growth, and angiogenesis. NF-kappaB transcriptionally upregulates NOLC1 and circNOLC1 via binding the NOLC1 promoter. |
| Confidence score: |
0.894 |