| Expression pattern: |
DN |
| Associated gene: |
KSRP, RUNX1, DHX9, pri-miR-320a, pre-miR-320a, Drosha, Dicer |
| Associated microRNA: |
hsa-miR-320a |
| Biological function: |
Suppresses CRC cell growth/proliferation and metastasis (migration and invasion); inhibits tumor growth in vivo and in PDO/PDX models. |
| Molecular mechanism: |
Acts as an RNA scaffold that enhances KSRP-mediated processing of pri-miR-320a and pre-miR-320a, promoting miR-320a maturation and repressing RUNX1; circ7379 biogenesis depends on RCMs and is negatively regulated by DHX9. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; RNA-seq; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Co-IP; Bioinformatics Analysis; Cohort Study |
| Clinical significance: |
Low circ7379 expression in CRC tissues is associated with larger tumor size, greater invasion depth, and advanced TNM stage; highlighted as a biomarker for predicting CRC progression. |
| Description: |
circ_0007379 (circ7379; hsa_circ_0007379) is down-regulated in colorectal cancer and functions as a tumor suppressor. It binds KSRP and serves as a scaffold to promote pri-/pre-miR-320a processing, increasing mature miR-320a and thereby repressing RUNX1 to inhibit CRC proliferation and metastasis, including in PDO/PDX and xenograft models. |
| Confidence score: |
0.898 |