circRNA basic information
circBase ID: hsa_circ_0000043
Name: hsa_circ_PUM1
Synonym: circPUM1
Host Gene: PUM1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004958
MONDO name: oral cavity squamous cell carcinoma
Disease details: oral squamous cell carcinoma / OSCC
Disease DO ID:
 0050866
Disease MeSH ID:
 -
Disease NCIt ID:
 C4833
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HSC-6; SCC-9; UM2; UM1; CAL-27; HSC-3; SCC-1; SCC-15; HOK
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: SP2, NAP1L1, AGO2
Associated microRNA: miR-770-5p
Biological function: promotes cisplatin chemoresistance and decreases susceptibility to NK cell-mediated cytotoxicity in OSCC cells
Molecular mechanism: SP2 transcriptionally induces circPUM1; circPUM1 acts as a miRNA sponge for miR-770-5p to regulate NAP1L1, thereby modulating cisplatin resistance and NK cell cytotoxicity in OSCC cells
Biological pathway or process:

immune regulation (other); chemoresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8
Clinical significance:

-
Description:

In OSCC cell lines, circPUM1 promotes cisplatin (CDDP) chemoresistance and reduces susceptibility to NK cell-mediated killing. Mechanistically, SP2 transcriptionally upregulates circPUM1, which sponges miR-770-5p to de-repress NAP1L1, forming an SP2/circPUM1/miR-770-5p/NAP1L1 axis.
Confidence score:

0.7366
Other information
Title:

SP2-induced circPUM1 modulates chemoresistance and nature killer cell toxicity in oral squamous cell carcinoma.

Journal: Journal of cellular and molecular medicine
Published: 2024
PubMed ID: 37556099
Study type:

biological research
Data availability: All data that support the findings of this study are available from the corresponding authors upon reasonable request.
Code availability: -