| Expression pattern: |
UP |
| Associated gene: |
LASP1, LIM and SH3 protein 1, E2F3 |
| Associated microRNA: |
miR-1287-5p |
| Biological function: |
Circ_0075804 promotes RB cell growth, proliferation, survival and invasion, inhibits apoptosis, and promotes tumor growth in vivo. |
| Molecular mechanism: |
Circ_0075804 acts through a ceRNA-like mechanism by targeting miR-1287-5p to positively regulate LASP1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Luciferase Reporter Assay; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Circ_0075804 had considerable diagnostic value for RB, with ROC AUC = 0.9081. |
| Description: |
Circ_0075804 is up-regulated in retinoblastoma tissues and cell lines and shows diagnostic potential. Functionally, circ_0075804 promotes RB cell proliferation, survival and invasion and supports xenograft tumor growth. Mechanistically, it targets miR-1287-5p to increase LASP1 expression, forming a circ_0075804/miR-1287-5p/LASP1 regulatory axis. |
| Confidence score: |
0.7892 |