| Expression pattern: |
UP |
| Associated gene: |
RNF219, CAV1, IL‐10RA |
| Associated microRNA: |
miR‐152‐3p |
| Biological function: |
promotes OA progression by enhancing oxidative stress and catabolic factor expression and suppressing anabolic genes in chondrocytes |
| Molecular mechanism: |
ceRNA sponge of miR‐152‐3p to de-repress RNF219, which stabilizes CAV1 by inhibiting its ubiquitination (K47), thereby inhibiting IL-10 signaling (via IL-10R/JAK/STAT3 changes) and promoting OA-associated oxidative stress |
| Biological pathway or process: |
oxidative phosphorylation (other); inflammation (promotes); ceRNA regulation (promotes); ubiquitination (inhibits); JAK/STAT (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; Co-IP; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; Flow Cytometry（Non-apoptosis/cycle）; Bioinformatics Analysis |
| Clinical significance: |
circGNB1 expression positively correlates with OA severity/degeneration and age in patient cartilage |
| Description: |
circGNB1 is up-regulated in osteoarthritic/aged cartilage and stressed chondrocytes and promotes OA phenotypes. It acts as a cytoplasmic ceRNA that sponges miR-152-3p to increase RNF219, which stabilizes CAV1 by reducing its ubiquitination (K47), thereby inhibiting IL-10 signaling (IL-10R/JAK/STAT3) and enhancing oxidative stress and cartilage catabolism. |
| Confidence score: |
0.8853 |