| Expression pattern: |
DN |
| Associated gene: |
FOXO3, RB1, CDK6, BCL2, PIK3R5, PLCG2 |
| Associated microRNA: |
hsa-miR-12119, hsa-miR-370-3p, hsa-miR-4539, hsa-miR-6511a-5p, hsa-miR-6772-3p, hsa-miR-9851-3p |
| Biological function: |
Potential biomarker of tumor hypoxia; may participate in lung cancer development and may reflect metastatic risk of lung cancer. |
| Molecular mechanism: |
Predicted circRNA-miRNA-mRNA regulatory mechanism involving targeted miRNAs and downstream mRNAs; GO and KEGG analyses suggest effects on signal transduction, protein phosphorylation, MAPK cascade, ERK1/2 cascade, and cancer-related pathways. |
| Biological pathway or process: |
ceRNA regulation (other); MAPK (other); ERK (other); PI3K/AKT (other); HIF-1 signaling (other); NF-kappaB (other); Wnt/beta-catenin (other); TGF-beta/SMAD (other); VEGF/VEGFR (other); apoptosis (other); migration (other); metastasis (other); proliferation (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; circRNA-seq; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Potential biomarker of hypoxic lung adenocarcinoma and tumor hypoxia; may have potential to predict metastatic risk of lung cancer. |
| Description: |
CircFAM120A is a human FAM120A-derived circRNA that is significantly downregulated in hypoxic A549 lung adenocarcinoma cells and lymph node-positive lung adenocarcinoma tissues. The study proposes CircFAM120A as a potential biomarker of lung adenocarcinoma hypoxia and metastatic risk, with predicted circRNA-miRNA-mRNA regulatory effects on signal transduction and cancer-related pathways. |
| Confidence score: |
0.6333 |