| Expression pattern: |
UP |
| Associated gene: |
MCT4, AGO2 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
Promotes proliferation, migration, aerobic glycolysis and tumor growth in bladder cancer. |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-330-5p to upregulate MCT4, thereby promoting glycolysis and bladder cancer progression. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High circ235 expression was correlated with poorer patient prognosis in bladder cancer. |
| Description: |
hsa_circ_0000235 (circ235) is upregulated in bladder cancer and promotes proliferation, migration, and aerobic glycolysis. Mechanistically, it functions as a ceRNA sponge for miR-330-5p to increase MCT4 expression, enhancing glycolytic metabolism and tumor growth, and higher circ235 correlates with worse prognosis. |
| Confidence score: |
0.8912 |