| Expression pattern: |
UP |
| Associated gene: |
CDKN2A mRNA, SMAD3 mRNA, YTHDC1, YTHDF2, AGO2 |
| Associated microRNA: |
miR-1197 |
| Biological function: |
promotes tumor cell proliferation and growth; promotes cell cycle progression; promotes tumor progression |
| Molecular mechanism: |
m6A-dependent cytoplasmic export via YTHDC1; direct circRNA-mRNA interaction promotes CDKN2A mRNA decay by recruiting YTHDF2; ceRNA sponging of miR-1197 to derepress SMAD3 |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes); m6A modification (promotes); mRNA stability (inhibits) |
| Detected method: |
Q
H
|
| Validation methods: |
FISH / smFISH; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis; MeRIP / MeRIP-seq |
| Clinical significance: |
high circMET correlates with higher tumor stage; link with prognosis not firmly established due to small sample size |
| Description: |
In NONO-TFE3 tRCC, circMET is up-regulated and promotes tumor cell proliferation and cell-cycle progression. Mechanistically, m6A-modified circMET is exported to the cytoplasm via YTHDC1, directly binds CDKN2A mRNA to recruit YTHDF2 and accelerate CDKN2A mRNA decay, and acts as a ceRNA sponging miR-1197 to upregulate SMAD3. |
| Confidence score: |
0.8165 |