| Expression pattern: |
UP |
| Associated gene: |
COL1A1, VDAC2, AGO2 |
| Associated microRNA: |
miR-577 |
| Biological function: |
Enhances migration and invasion (metastatic ability) of RCC cells; activates EMT; increases ferroptosis sensitivity (especially to erastin). |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-577 to regulate downstream targets COL1A1 (EMT activation) and VDAC2 (ferroptosis sensitivity). |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); ferroptosis (promotes); ceRNA regulation (other); migration (promotes); invasion (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; Transwell Assay; CCK8; IF (Immunofluorescence); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Rescue experiments |
| Clinical significance: |
Higher hsa_circ_0057105 expression is associated with advanced TNM stages and higher Fuhrman grade, and is an independent prognostic factor for poor OS and RFS in ccRCC patients. |
| Description: |
In RCC/ccRCC, hsa_circ_0057105 is up-regulated and mainly localized in the cytoplasm. It promotes migration/invasion and EMT while simultaneously increasing erastin-related ferroptosis sensitivity by sponging miR-577 and derepressing COL1A1 and VDAC2. High expression correlates with advanced stage and predicts poorer OS/RFS. |
| Confidence score: |
0.8981 |